|Director: Arlene H. Sharpe, M.D., Ph.D.
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77 Avenue Louis Pasteur
Brigham and Women's Hospital
Department of Dermatology
Boston, MA 02115
1. K14/IL-1 Alpha Line 2; K14 promoter/enhancer targeting overexpression of the 17 kD form of mouse IL-1 alpha to basal keratinocytes. Overproductionof IL-1alpha, a protoype primary cytokine, by keratinocytes leads to induction of numerous secondary cytokines, induction of endothelial cell adhesion molecules, and inflammatory skin disease.
Reference: Groves, R.W., H. Mizutani, J.D. Kieffer, and T.S. Kupper.1995. Inflammatory skin disease in transgenic mice that express high levels of interleukin-1 alpha in basal epidermis. Proc. Natl. Acad. Sci. USA, 92:11874-11878.
2. K14/IL-1R type I Line 1R10; K14 promoter/enhancer targeting overexpressionof the mouse type I IL-1 receptor to basal keratinocytes. Overexpression of the signal transducing IL-1 receptor by keratinocytes leads to heightened susceptibility to cutaneous inflammation in response to IL-1 or IL-1-inducing stimuli.
Reference: Groves R.W., T. Rauschmayr, K. Nakamura, S. Sarkar, I.R.Williams, and T.S. Kupper. 1996. Modulation of induced inflammation in transgenic mice that overexpress type-1 IL-1 receptor in epidermis. Evidence for cutaneous IL-1 mediated autocrine feedback pathways in vivo. J. Clin. Invest. 98:336-344.
3. K14/IL-1R type II; K14 promoter/enhancer targeting overexpression of the mouse type II IL-1 receptor to basal keratinocytes.
Reference: Rauschmayr, T., R.W. Groves, and T.S. Kupper. 1997. Keratinocyte expression of the type 2 interleukin 1 receptor mediates local and specific inhibition of interleukin 1-mediated inflammation. Proc. Natl. Acad. Sci.USA 94:5814, 1997.
4. K14/IL-1Ra; K14 promoter/enhancer targeting overexpression ofmouse interleukin-1 receptor antagonist to basal keratinocytes.
Reference: Groves, R.W., I.R. Williams, S. Sarkar, K. Nakamura, andT.S. Kupper. 1994. Analysis of epidermal IL-1 family members in vivo usingtransgenic mouse models. J. Invest. Dermatol. 102:556 (abstract).
5. K14/IL-1 alpha pro
6. K14/IL-12 p40; K14 promoter/enhancer targeting overexpression of the p40 chain of mouse IL-12 to basal keratinocytes.
7. K14/IL-7; K14 promoter/enhancer targeting overexpression of mouse IL-7 to basal keratinocytes. Keratinocyte overexpression of the T cell growthfactor IL-7 leads to increased mobilization of a population of activatedgamma/delta T cells to the skin. The increased T cell content of the skin in turn leads to a drastic hyperresponsiveness (with as much as 100-foldincrease in sensitivity) to a variety of irritant stimuli applied to skin.
Reference: Williams, I.R., E.A. Rawson, L. Manning, T. Karaoli, B.E.Rich, and T.S. Kupper. 1997. IL-7 overexpression in transgenic mouse keratinocytescauses a lymphoproliferative skin disease dominated by intermediate TCRcells. Evidence for a hierarchy in IL-7 responsiveness among cutaneous T cells. J. Immunol. 159:3044-3056.
8. Ig/IL-7; Immunoglobulin promoter and enhancer directing the expression of IL-7 to lymphocytes. These mice devlop a progressive cutaneous lymphoproliferaive disorder that progresses to malignancy and stochastic lymphomas.
Reference: Rich, B.E, J. Campos-Torres, R.I. Tepper, R.M. Moreadith and P. Leder (1993) Cutaneouus Lymphoroliferation and Lymphomas in Interleukin 7 Transgenic Mice. J. Exp. Med. 177:305-316
9. IL-7 KO (Balb/c)
10. IL-7 KO (FVB/N)
11. IL4-EGFP Knock-in
T cell receptor transgenes
OT-1 Thy1.2 (C57BL/6)
OT-1 Thy1.1 (C57BL/6)
OT-1 Rag-2 KO Thy 1.1
OT-1 Fucosyltransferase IV/VII double KO
OT-1 x BoyJ
OT-1 x CCR4 KO
OT-1 x CCR9 KO
OT-2 CD45.1 Rag -/-
OT-2 x BoyJ
OT-2 x CCR4 KO
OT-2 x CCR9 KO
OT-2 x BoyJ x CCR4 KO
OT-2 x BoyJ x CCR10 KO
OT-2 x Fucosyltransferase IV/VII double KO
Mice with immunologic defects
RAG-2 KO (FVB/N)
RAG-2 JAK-3 double KO (FVB/N)
beta-2 microglobulin KO
Invariant Chain KO
Rag 1 KO
RAG 1 KO x CCL25 KO
Mice expressing ovalbumin
Mice expressing fluorescent proteins
Foxp3 - GFP
Foxp3-GFP OT II CD45.1